Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique
نویسندگان
چکیده
منابع مشابه
Comparison of immunoglobulin determinations in pathological sera by radial immunodiffusion and laser nephelometry.
Immunoglobulins G, A, and M were measured by radial immunodiffusion and by laser nephelometry in sera from 33 patients and in a preparation of purified colostral IgA. In general the results agreed for the two methods, with some exceptions attributed to the physicochemical characteristics of the immunoglobulins assayed: low-molecular-weight (monomeric) IgM gives falsely high values and IgA prote...
متن کاملLaser nephelometry and radial immunodiffusion compared for immunoglobulin quantification in pathological sera.
We evaluated nephelometers from Behring, Hyland, and Beckman for IgG, IgA, and IgM quantitation in sera from patients with monoclonal gammopathies. The intra-batch precision of each instrument for each immunoglobulin class and for different concentrations of the same immunoglobulin was compared to the one obtained with the radial immunodiffusion method. No nephelometer showed a clearly better p...
متن کاملConcentrations of plasma immunoglobulins in the dog as determined by laser nephelometry. Comparison with radial immunodiffusion and enzyme-linked immunosorbent assay.
Concentrations of immunoglobulins were determined by laser nephelometry and, for comparison, by single radial immunodiffusion assay and a sandwich enzyme-linked immunosorbent assay (ELISA) in plasma samples of normal dogs. Correlation between methods was high, although the mean IgM concentrations determined by laser nephelometry were significantly higher than those determined by the single radi...
متن کاملComparison of radial immunodiffusion and laser nephelometry for quantitating some serum proteins.
After quantitating immunoglobulins G, A, and M and complement C3c and C4 in serum by using a laser nephelometer coupled with a data processor, I compared these results with values obtained by an early-readout radial immunodiffusion method. Day-to-day precision was better for nephelometry than for radial immunodiffusion for all proteins analyzed. The average coefficient of variation was 6.0% for...
متن کاملComparison of protein values in cerebrospinal fluid by nephelometry and radial immunodiffusion.
Various parameters of three radial immunodiffusion systems, a kinetic nephelometer and two laser nephelometers were compared in a measurement of IgG and albumin in cerebrospinal fluid. Greater precision for IgG and albumin determinations was found with two RID systems. Correlation coefficients between methods ranged from 0.71 to 0.94. The generally poor correlations of methods, combined with th...
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ژورنال
عنوان ژورنال: Veterinary Immunology and Immunopathology
سال: 1980
ISSN: 0165-2427
DOI: 10.1016/0165-2427(80)90006-9